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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and thus might add to tumorigenesis. This protein was located to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a vital function in advertising cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two supplemental splice variants ended up noted but in-depth nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind especially to the focus on antibody and block antibody binding. These peptide commonly includes the epitope identified by the antibody.
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This guidebook outlines even further doable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This manual outlines further more doable optimization of mobile and immunoassay parameters to guarantee the best possible final results are...
Antibodies certain to the blocking peptide not bind on the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.
Focus on Details Cyclin E belongs for the extremely conserved cyclin family members, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add into the temporal coordination of each mitotic occasion. Cyclin E forms a posh with and functions for a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and it is degraded as cells progress by S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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